Sense organs

Precise measurement of receptor thresholds is important in understanding
the role a sense organ plays in the biology and the sensory information
processing of an organism. Previous crustacean chemoreceptor thresholds
are too high because of the adaping effects of high stimulus intensities
and the order effects of stimulus presentation sequence used in threshold
experiments now in the literature. The present study uses an experimental
design geared to produce the lowest adaptation possible and shows
that single unit chemoreceptor activity occurs at lower concentrations
than any previously documented. The differences between lateral and
medial chemoreceptor and the variability of the antennular chemoreceptor
population are also treated in the present study. It is concluded that
threshold differences do not account for the differential behavioral
effect of lateral vs medial antennal filament ablation, and that the
observed response variability is inherent in receptor response and
important to receptor function.

Elasmobranch fishes use electroreception to detect electric fields in the environment, particularly minute bioelectric fields produced by potential prey. A single elasmobranch family (Potamotrygonidae) is composed of obligate freshwater stingrays endemic to the Amazon River. A freshwater existence has imposed morphological adaptions on their electrosensory system due to life in a high impedance medium. Because their electrosensory morphology differs from their marine relatives, freshwater stingrays may demonstrate corresponding differences in behavioral sensitivity. The objective of this study was to quantify behavioral sensitivity of the obligate freshwater stingray Potamotrygon motoro to prey-simulating voltage. The voltage produced by common teleost prey of P. motoro were measured and replicated for behavioral trials. The best response was 10.62 cm, and the smallest voltage gradient detected was 0.005 mVcm-1. This sensitivity is reduced compared to marine species. The conductivity of the medium, more so than ampullary morphology, may dictate sensitivity of the elasmobranch electrosensory system.

The olfactory system is the most highly developed system for molecular sensing in vertebrates. Despite their reputation for being particularly olfactory driven, little is known about how this sense functions in elasmobranch fishes. The goal of this dissertation was to examine the morphology and physiology of elasmobranchs to compare their olfactory system with teleost fishes and more derived vertebrates. To test the hypotheses that elasmobranchs possess greater olfactory sensitivities than teleosts and that lamellar surface area is correlated to sensitivity, I compared the surface area of the olfactory lamellae and the olfactory sensitivities of five phylogenetically diverse elasmobranch species. The olfactory thresholds reported here (10-9 to 10-6 M) were comparable to those previously reported for teleosts and did not correlate with lamellar surface area. Since aquatic species are subject to similar environmental amino acid levels, they appear to have converged upon similar amino acid sensitivities. To test the hypothesis that elasmobranchs are able to detect bile salt odorants despite lacking ciliated olfactory receptor neurons (ORNs), the type of ORN that mediates bile salt detection in the teleosts, I quantified the olfactory specificity and sensitivity of two elasmobranch species to four, teleost-produced C24 bile salts. Both species responded to all four bile salts, but demonstrated smaller relative responses and less sensitivity compared to teleosts and agnathans. This may indicate that elasmobranchs don't rely on bile salts to detect teleost prey. Also, the olfactory system of elasmobranchs contains molecular olfactory receptors for bile salts independent of those that detect amino acids, similar to teleosts.