Model
Digital Document
Publisher
Florida Atlantic University
Description
tmRNA is a small stable RNA present in Eubacteria. Through a mechanism called
trans-translation, tmRNA mediates ribosome rescue and quality control of proteins and
mRNA. In this study, the Escherichia coli (E. coli) mutant lacking tmRNA was
demonstrated hypersensitive to oxidative stress. The role of tmRNA-mediated
surveillance mechanism in protecting E. coli cell under oxidative stress condition was
examined. The tmRNA-mediated tagged protein levels were elevated in cells under
oxidative stress condition, demonstrating the enhanced need for tmRNA under such
condition. Our results suggest that mRNA damage by oxidative stress may cause reduced
cell viability, and that tmRNA is required to rescue cells under such condition.
Furthermore, our observations showed that tmRNA is required for the optimal growth of
E. coli under normal aeration but not under anaerobic condition, suggesting that oxidation
ofmRNA is the major reason for requirement oftmRNA during normal aeration.
trans-translation, tmRNA mediates ribosome rescue and quality control of proteins and
mRNA. In this study, the Escherichia coli (E. coli) mutant lacking tmRNA was
demonstrated hypersensitive to oxidative stress. The role of tmRNA-mediated
surveillance mechanism in protecting E. coli cell under oxidative stress condition was
examined. The tmRNA-mediated tagged protein levels were elevated in cells under
oxidative stress condition, demonstrating the enhanced need for tmRNA under such
condition. Our results suggest that mRNA damage by oxidative stress may cause reduced
cell viability, and that tmRNA is required to rescue cells under such condition.
Furthermore, our observations showed that tmRNA is required for the optimal growth of
E. coli under normal aeration but not under anaerobic condition, suggesting that oxidation
ofmRNA is the major reason for requirement oftmRNA during normal aeration.
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