Model
Digital Document
Publisher
Florida Atlantic University
Description
Extracellular stimuli may influence the M1/M2 phenotypic polarization of
macrophages. We examined M1/M2 biomarkers, phagocytic activity, and tumoricidal
activity in RAW 264.7 mouse macrophages. Macrophages were treated with conditioned
media (CM) from 4T1 breast cancer cells, curcumin, 22-oxacalcitriol, LPS, or a
combination of the previously listed. Arginase activity, a M2 phenotypic biomarker, was
upregulated by the treatment of macrophages with conditioned media. Curcumin, 22-
oxacalcitriol, and LPS partially inhibited RAW 264.7 arginase activity in the presence of
4T1 breast cancer media. 22-oxacalcitriol increased the phagocytic ability of RAW 264.7
macrophages in the presence of M2 polarizing substances produced by the 4T1 breast
cancer cells. Also, LPS increased RAW 264.7 phagocytic ability in the presence of 4T1
breast cancer CM. This study looked at the potential substances that would possibly reverse the M2 tumor promoting macrophage phenotype seen in the breast cancer tumor
environment.
macrophages. We examined M1/M2 biomarkers, phagocytic activity, and tumoricidal
activity in RAW 264.7 mouse macrophages. Macrophages were treated with conditioned
media (CM) from 4T1 breast cancer cells, curcumin, 22-oxacalcitriol, LPS, or a
combination of the previously listed. Arginase activity, a M2 phenotypic biomarker, was
upregulated by the treatment of macrophages with conditioned media. Curcumin, 22-
oxacalcitriol, and LPS partially inhibited RAW 264.7 arginase activity in the presence of
4T1 breast cancer media. 22-oxacalcitriol increased the phagocytic ability of RAW 264.7
macrophages in the presence of M2 polarizing substances produced by the 4T1 breast
cancer cells. Also, LPS increased RAW 264.7 phagocytic ability in the presence of 4T1
breast cancer CM. This study looked at the potential substances that would possibly reverse the M2 tumor promoting macrophage phenotype seen in the breast cancer tumor
environment.
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