Research

This poster presentation will illustrate how digital collections add value to the scholarly communication chain by supporting research, teaching, and scholarship in several ways: 1) increase access to primary materials, 2) increase access to special collections and archives 3) increase access to local materials of historical, cultural, and artistic significance 4) expand open access 5) foster collaboration with faculty and students 6) increase the reputation and visibility of your university and library. I will use specific examples from the Florida Atlantic University Digital Collections to “show and tell” and include the importance of creating metadata to enhance discovery and access to your digital collections. In addition, this poster will very briefly touch upon digital sustainability; mainly that of ensuring ongoing access to digital collections and ensuring long term preservation of these same materials.

The projects described in this dissertation are focused on compounds derived from the marine environment. Chapter 1 gives an introduction to the study of marine natural products to treat human ailments and a thorough review on compounds from lithistid sponges that have been isolated or synthesized since 2000. Chapter 2 describes the isolation and structure elucidation of two sesquiterpene substituted benzoquinone derivatives, petrosiquinones A and B, from a deep-water marine sponge from the Family Petrosiidae. Although initially purified following activity in a (Sb(B-catenin/Tcf4 assay they were later followed using tumor cell line cytotoxicity assays. Petrosiquinone A was the more active of the two compounds with moderate cytotoxicity in the DLD-1, PANC-1, and AsPC-1 cell lines. In Chapter 3, the isolation and structure elucidation of two new marine-derived macrolides, madeirolide A and B, isolated from a deep-water lithistid sponge of the genus Leiodermatium is described.

This study investigated electroencephalographic differences related to cue (central left- or right-directed arrows) in a covert endogenous visual spatial attention task patterned after that of Hopf and Mangun (2000). This was done with the intent of defining the timing of components in relation to cognitive processes within the cue-target interval. Multiple techniques were employed to do this. Event-related potentials (ERPs) were examined using Independent Component Analysis. This revealed a significant N1, between 100:200 ms post-cue, greater contralateral to the cue. Difference wave ERPs, left minus right cue-locked data, divulged significant early directing attention negativity (EDAN) at 200:400 ms post-cue in the right posterior which reversed polarity in the left posterior. Temporal spectral evolution (TSE) analysis of the alpha band revealed three stages, (1) high bilateral alpha precue to 120 ms post-cue, (2) an event related desynchronization (ERD) from approximately 120 ms: 500 ms post-cue, and (3) an event related synchronization (ERS) rebound, 500: 900 ms post-cue, where alpha amplitude, a measure of activity, was highest contralateral to the ignored hemifield and lower contralateral to the attended hemifield. Using a combination of all of these components and scientific literature in this field, it is possible to plot out the time course of the cognitive events and their neural correlates.

Metastatic cancers are problematic because they spread throughout the body. A crucial step in cancer metastasis is the separation of the cancer cells from their surrounding normal cells. This occurs due to suppression or destruction of cell adhesion molecules such as E-cadherin, occludin, and various claudins. The Snail and Slug transcription factors play a direct role in suppressing these cell adhesion molecules through their SNAG repression domain. We explored the possibility of developing an ELISA diagnostics capable of detecting soluble E-cadherin, occludin, and claudin fragments in the serum of cancer patients. Using several bioinformatics tools, unique extracellular antigenic sequences were identified on claudins-1, 4, 16, occludin, and E-cadherin. These sequences were cloned as GST fusion proteins, expressed, and purified in large quantities to raise antibodies. In parallel, expression profiling of metastatic cancer cell lines was carried out to derive a correlation between Snail-Slug expression and suppression of cell adhesion molecules.

This study investigates affect coding within the therapeutic relationship, by exploring the client's and therapist's perception of the relationship and the facial and vocal affect expressed by both parties. A sample of 14 therapy sessions each having 1800 data points was collected. The Working Alliance Inventory Short Form (WAI-S) and Real Relationship Inventory (RRI) were completed after each recorded session. The participants were therapists and clients at a university counseling center in South Florida. Data were analyzed using one-tailed t tests, descriptive statistics, scores from RRI and the WAI-S and percentages of negative, neutral and positive affect. Statistically significant relationships were found between seconds of therapist negative affect (t(13)= -2.065, p. <.05) and seconds of therapist neutral affect (t(13)= -1.959, p. <.05) for clients who dropped out of therapy. The seconds of negative affect coded for clients (t(13) = -1.396, p. >.05) was approaching statistical significance for clients who drop out of therapy. This study provides theoretical and empirical support for linking the presence of facial affect in the first session and its effects on the therapeutic relationship and thus client retention or drop out. The clinical implications of these findings are also discussed.

The lens is an avascular organ that focuses light onto the retina where neural signals are transmitted to the brain and translated into images. Lens transparency is vital for maintaining function. The lens is formed through a transition from organelle-rich epithelial cells to organelle-free fiber cells. Lens cell differentiation, leading to the lack of organelles, provides an environment optimal for minimizing light scatter and maximizing the ability to focus light onto the retina. The process responsible for orchestrating lens cell differentiation has yet to be elucidated. In recent years, data has emerged that led our lab to hypothesize that autophagy is likely involved in lens cell maintenance, cell differentiation, and maintenance of lens transparency. As a first step towards testing this hypothesis, we used RT-PCR, western blot analysis, immunohistochemistry, confocal microscopy, and next generation RNA-Sequencing (RNA-Seq) to examine autophagy genes expressed by the lens to begin mapping their lens function.

With antimicrobial resistance to current drugs steadily rising, the development of new antibiotics with novel mechanisms of action has become an imperative. The majority of life-threatening infections worldwide are caused by "ESKAPE" pathogens which are encountered in more than 40% of hospital-acquired infections, and are resistant to the majority of commonly used antibiotics. Naturally occurring cyclic depsipeptides, microbial secondary metabolites that contain one or more ester bonds in addition to amide bonds, have emerged as an important source of pharmacologically active compounds or lead structures for the development of novel antibiotics. Some of those peptides are either already marketed (daptomycin) or in advanced stages of clinical development (ramoplanin). Structurally simple, yet potent, fusaricidin/LI-F and lysobactin families of naturally occurring antibiotics represent particularly attractive candidates for the development of new antibacterial agents capable of overco ming infections caused by multidrug-resistant bacteria. These natural products exhibit potent antimicrobial activity against a variety of clinically relevant fungi and Gram-positive bacteria. Therefore, access to these classes of natural products and their synthetic analogs, combined with elucidation of their mode of action represent important initial steps toward full exploitation of their antmicrobial potential. This dissertation describes a general approach toward the solid-phase synthesis of fusaricidin/LI-F and lysobactin analogs and an extensive structure-activity relationship (SAR) study. We have devised a simple and robust preparation strategy based on standard Fmoc solid-phase peptide synthesis protocols.

Diabetic retinopathy is an ischemic retinal neovascular disease causing vision loss among adults. The studies presented involve the design and testing of a gene therapy vector to inhibit retinal revascularization, similar to that found in diabetic retinopathy. Gene therapy has proven to be an effective method to introduce therapeutic proteins to treat retinal diseases. Targeting a specific cell type and expression of therapeutic proteins according to the tissue microenvironment should have an advantage over traditional gene therapy by avoiding unwanted transgene expression. Hypoxia plays a significant role in the pathophysiology of many retinal ischemic diseases. Retinal Mèuller cells provide structural and functional support to retinal neurons, as well as playing a significant role in retinal neovascularization. Targeting Mèuller cells may be an effective strategy to prevent retinal neovascularization under pathological conditions. ... The hypoxia regulated, glial specific vector successfully reduced the abnormal neovascularization in the periphery by 93% and reduced the central vasobliterated area by 90%. A substantial amount of exogenous endostatin was produced in the retinas of P17 OIR mice. A significant increase in human endostatin protein and reduced vascular endothelial growth factor (VEGF) were identified by Western blot and ELISA, respectively. These findings suggest hypoxia-regulated, glial cell-specific scAAV mediated gene expression may be useful to prevent blindness found in devastating retinal diseases involving neovascularization.

Despite extensive research into memory and violence, relatively little is known about the relationship between violent media and memory of advertised products. Research has yielded contradictory evidence ; some scholars have found a negative relationship, others a nonexistent one... This research investigated the role of emotion in the relationship between violent media and product placement memory. This study creates insight into inconclusive previous findings by providing evidence that violence influences product memory. Specifically, participants were significantly worse at remembering products placed within violent clips (e.g., free recall, cued recall, recognition. Participants' emotional responses to the violent clips also appeared to influence their memory for embedded products ; product recognition was significantly correlated with disgust, avoidance, and interest ratings.... Interestingly, stronger negative responses to the violent clips were correlated with decreased preference for the embedded products. Furthermore, the pattern of differences for product preference between target and non-target violent products varied according to negative emotional reaction. Therefore, this dissertation provides insight into the role emotion plays in the relationship between viewing violent media and product placement memory.

Simulations have been carried out to validate a hydrokinetic energy system non-dimensional scaling procedure. The requirements for a testing facility intended to test such devices will be determined from the results of the simulations. There are 6 simulations containing 3 prototype systems and 2 possible model facility depths to give a range of results. The first 4 tests are conducted using a varying current profile, while the last 2 tests use a constant current profile of 1.6 m/s. The 3 prototype systems include a: 6 m spherical buoy, a 12 m spherical buoy and a turbine component system. The mooring line used for the simulations is a 6x19 Wire Rope Wire Core of diameter 100 mm and length 1000 m. The simulations are implemented using Orcaflex to obtain the dynamic behavior of the prototype and scaled system.