Model
Digital Document
Publisher
Florida Atlantic University
Description
Troponin I is a contractile protein and plays an important role in cardiac function.
We have generated cTnl knockout and cTnI(R192H) transgenic mouse models. All of
cTnl knockout homozygous mice die at 17-18 days after birth. Some of cTnI(R192H)
transgenic mice die at early life stages, some mice develop heart failure at late stages.
High-resolution ultrasound imaging and Doppler echocardiography have been used to
evaluate cardiac function on cTnl deficient mice and cTnl(R192H) transgenic mice.
cTnI mice have damaged relaxation with gradually decreased E/A ratio(E/A<1). FS
and cardiac output dramatically decrease on 17-day-o1d cTnI mice indicating severe
cardiac dysfunction. We find that the damaged heart function is correspondent with the
Tnl expression level decline. 6-8 weeks transgenic mice have shown that the dimension
of left and right atria increase. In 15-month-old transgenic mice, the E/A ratio shows a
pseudonormal pattern indicating a diastolic dysfunction. This study demonstrate that
damaged heart function is tightly associated with Tnl levels in the heart.
We have generated cTnl knockout and cTnI(R192H) transgenic mouse models. All of
cTnl knockout homozygous mice die at 17-18 days after birth. Some of cTnI(R192H)
transgenic mice die at early life stages, some mice develop heart failure at late stages.
High-resolution ultrasound imaging and Doppler echocardiography have been used to
evaluate cardiac function on cTnl deficient mice and cTnl(R192H) transgenic mice.
cTnI mice have damaged relaxation with gradually decreased E/A ratio(E/A<1). FS
and cardiac output dramatically decrease on 17-day-o1d cTnI mice indicating severe
cardiac dysfunction. We find that the damaged heart function is correspondent with the
Tnl expression level decline. 6-8 weeks transgenic mice have shown that the dimension
of left and right atria increase. In 15-month-old transgenic mice, the E/A ratio shows a
pseudonormal pattern indicating a diastolic dysfunction. This study demonstrate that
damaged heart function is tightly associated with Tnl levels in the heart.
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