Model
Digital Document
Publisher
Florida Atlantic University
Description
The composition of marine bacterial communities from South Florida beaches
were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured
populations, community genomic DNA was extracted directly from sand and
seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct
extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in
Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70),
the populations cluster into several unknown clads with early divergence, indicating the
presence of novel well established bacterial groups. Members of phylum Bacteroidetes,
Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in
culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach.
Taxonomic representation indicates marine water as more diverse compared to dry sand
and wet sand. A combination of phylogenetic markers will be needed to define the
immense diversity of this niche.
were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured
populations, community genomic DNA was extracted directly from sand and
seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct
extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in
Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70),
the populations cluster into several unknown clads with early divergence, indicating the
presence of novel well established bacterial groups. Members of phylum Bacteroidetes,
Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in
culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach.
Taxonomic representation indicates marine water as more diverse compared to dry sand
and wet sand. A combination of phylogenetic markers will be needed to define the
immense diversity of this niche.
Member of