Aging

Data was provided by researchers of the Einstein Aging Study (EAS) of the Albert Einstein College of Medicine, Yeshiva University whom statistically analyzed data from the Bronx Aging Study cohort, concluding that participation in cognitive leisure activities and one physical activity, dancing, were associated with a reduced risk of dementia [1]. We explore data from a second (the EAS) cohort, utilizing Cox Proportional-Hazards and extended Cox regression [13]. Cognitive leisure activities in general, and particularly doing crossword puzzles, reading books, watching television, and emailing are associated with a reduced risk of dementia. Doing aerobics, learning computer programming, babysitting, dancing, jogging singing, and weight training are associated with an increased risk of dementia. Participation in cognitive leisure activities in general, and reading books in particular, remains highly significant even after adjustment for well-known risk factors [14] such as: age, cognitive status, depression, medical illnesses, gender, ethnicity, education and economic status.

Age-related macular degeneration (AMD) is the leading cause of blindness in the western world for people over 60 years of age. The most severe pathological event of AMD is choroidal neovascularization (CNV), the process of new vessel formation emerging from the choroid. The new vessels extend into the normally avascular photoreceptor cell layer, where they leak fluid and cause photoreceptor cell death. CNV is thought to be initiated by hypoxia and chronic inflammation, which occur due to abnormal, age-related changes within the retinal pigmented epithelium (RPE). These events cause increased expression of the angiogenic protein vascular endothelial growth factor (VEGF) via hypoxiainducible factor-1 (HIF-1), a transcription factor that is vital in regulation of cellular responses to hypoxic and inflammatory conditions. Increased VEGF signaling stimulates proliferation and migration of vascular endothelial cells and facilitates the neovascular process. To target the early pathological events that lead to CNV, we have engineered a novel gene therapy vector that uses HIF-1 regulation to stimulate production of an angiostatic protein, endostatin from the RPE. The purpose of this study was to characterize the activity of our hypoxiaregulated, RPE-specific promoter in vitro, and investigate the effects of regulated endostatin expression, driven by our regulated promoter, on CNV in a mousemodel. We found the regulated promoter construct has robust activity in vitro only in RPE cells, and is conditionally responsive in hypoxic conditions.

The enzyme Methionine sulfoxide reductase A (MsrA) repairs oxidized proteins, and may act as a scavenger of reactive oxygen species (ROS), making it a potential therapeutic target for age-related neurodegenerative diseases. The anoxia-tolerant turtle offers a unique model to observe the effects of oxidative stress on a system that maintains neuronal function following anoxia and reoxygenation, and that ages without senescence. MsrA is present in both the mitochondria and cytosol, with protein levels increasing respectively 3- and 4-fold over 4 hours of anoxia, and remaining 2-fold higher than basal upon reoxygenation. MsrA was knocked down in neuronally-enriched cell cultures via RNAi transfection. Propidium iodide staining showed no significant cell death during anoxia, but this increased 7-fold upon reoxygenation, suggesting a role for MsrA in ROS suppression during reperfusion. This is the first report in any system of MsrA transcript and protein levels being regulated by oxygen levels.

In light of exceptionally delayed reproductive senescence exhibited by a 64 year old female chimpanzee (Pan troglodytes) housed in Florida, endocrinal analyses meant to determine the state of her current reproductive viability were conducted. Urine was collected from the study subject for a period of 88 days spaced within an interim of roughly 6 months and the specimens were sent to the Hominoid Reproductive Ecology Laboratory for assessment. Additional data was collected from three control females in order to provide a basis of comparison against the hormonal markers present in the geriatric study animal. Results indicate that the geriatric female does not presently appear to be cycling, but nor does she exhibit signs of complete reproductive cessation. This could signify that Pan troglodytes adheres to a pattern of reproductive aging not necessarily shared by Homo sapiens, which has further implications for the evolutionary trajectory of menopause in the human female.

This study examines whether obesity affects the accuracy of estimating age-at-death as measured by the age-related changes of the pubic symphysis and auricular surfaces. I scored the hip bones of 119 adults of normal body mass and 126 obese adults (total n = 254) using the SucheyBrooks (1990) method for the pubic symphysis and the Buckberry and Chamberlain (2002) method for the auricular surface. Compared to normal weight individuals, obese individuals exhibited greater inaccuracy in age-at-death estimates when aged from the auricular surface, but not the pubic symphysis. However, age was estimated with less precision in obese individuals using both methods. Obese males are more likely to be aged inaccurately than obese females. The pubic symphysis method may be the preferred method when estimating age in obese individuals, especially males, but forensic anthropologists should use caution when assessing age-at-death in obese adults using either method.

Healthy aging has been associated with declines in executive functioning (EF) but it remains unclear how different subprocesses of EF are affected by age and by other possibly mediating variables. The principal aim of the present study was to investigate the effects of age and processing speed on three executive functions: set-shifting, planning, and attentional control. Four age groups (20-29 years, 60-69 years, 70-79 years, and 80-89 years) were compared on the Wisconsin Card Sorting Test (WCST), the DKEFS Tower Test, the Conner's Continuous Performance Test, and a Letter comparison test of processing speed. Results suggested that increased age was associated with decreased performance on most of the studied executive measures, but not all EF are equally affected by age. A slowdown in processing speed mediates some, but not all, decrements in executive performance. The results are interpreted in light of recent neuroimaging data on age-related changes in brain functioning.

It was estimated that 35 million people age 65 or older lived in the United States in 2000. Of that number 2.8 million were Black/African American. The U.S. Census Bureau's (2000) population projections show that there will be 70 million older adults age 65 or older by 2030 and African Americans are expected to comprise over 12% of that population. In 1993 older adults had made less elective use of computers than younger adults, accounting for 24.2% of those age 55 to 64 and 4.9% of adults over age 65. By 2003 adults over age 65 recorded a 20.1% increase in computer usage becoming the fastest growing segment of computer users who are engaging in learning computer skills as a way of coping with the technological changes. Studies have found that greater experience with computers is associated with more positive attitudes; however, it has never been determined whether this is true of the older African American population since there is a paucity of research documenting their computer attitudes. This study utilized a mixed methods research design that included an experimental design and an inductive approach with interviews. The following findings emerged: (a) attitudes differed for older African Americans who received computer training and those who did not; (b) there was no distinction in computer attitudes between older adult male and older adult females in the African American population; (c) there was no interaction effect on computer attitudes as moderated by training and gender; (d) older African Americans exhibited a positive disposition towards computers which elicited positive attitudes towards the technology; (e) older African Americans had a nascent need for computer self-efficacy; and (f) older African Americans constructed new meaning regarding computers as a result of their reflection on their computer interaction experience.

The graying of America as a sociological and health care phenomenon continues to dictate the demand for creative and innovative interventions that can directly influence the quality of life of nursing home residents. Creative Reminiscence is a program that fosters the active reliving of the past through narration, such as in story-telling, and the use of creative art expression to uncover meaningful life events or valuable life experiences. The purpose of this study was to investigate and describe the meaningful life events elicited by the experience of Creative Reminiscence using a phenomenographic approach. Existing (archived) data-transcribed interviews, poetry and painting on planters that were obtained from 20 participants of the Creative Reminiscence program entitled Le Jardin de Sante were analyzed. Five variations of meaningful memories evoked by Creative Reminiscence were identified. These are described as follows: (a) memories that rejuvenate the elder's sense of connectio n; (b) memories that revive the elder's sense of love and intimacy; (c) memories that restore the elder's sense of being; (d) memories that reassert the elder's sense of control; and (e) memories that reinstitute the elder's sense of purpose. In a caring interaction with Creative Reminiscence, the nursed are able to transcend, moving back in time (past) to appreciate segment(s) of their lives, with triumphs and disappointments that give meaning to their inner conflict/human struggle (present), and integrate a sense of confidence and acceptance of their lives to sustain well-being and a sense of wholeness. This research highlights the similarities and differences in the description of memories by the nursing home residents evoked during Creative Reminiscence.

Two separate groups of young and middle-aged adults watched videos of events being performed by a number of different college-aged females. Both the young goup of adults and the middle-aged group of adults were later tested on their memory for both the individual features of these events (i.e., actors and actions), and for conjunctions of features (i.e., which actor performed which action) in order to determine how likely they were to remember which actors performed which actions. Our results showed that frontal lobe functioning is not affected with age. However, there is a slight decrease in medial temporal functioning that continues on throughout life. There was a main effect of Question, Item Type, and Age Group explained through a three-way ANOVA. Binding errors increase with age because specific areas of the brain, such as the hippocampus and prefrontal cortex, deteriorate with age.

Caloric restriction (CR), the reduction of nutrient intake short of malnutrition, extends the lifespan of various organisms and can improve measures of human health. Whether mechanisms of lifespan extension are conserved between humans and model organisms is unknown. In mammals, implementing CR is easily achieved by providing a restricted group with a fraction of the food consumed by an "ad libitum" fed group, which has unlimited food access. Due to the difficulty in directly controlling Drosophila food intake, caloric restriction, performed similarly to the mammalian paradigm, has never been tested in flies. Here, we demonstrate a system that allows measurement of food intake throughout life. This system will be used to measure fly lifespan under caloric restriction analogous to current mammalian studies. Our work will help tease apart the differences between the various caloric and dietary restriction paradigms in Drosophila, strengthening our understanding of how fly models relate to mammalian systems.