Ecosystem management

In plants, phenotypic plasticity, the ability to morphologically adapt to new or broad environmental conditions, is a consequence of long-term evolutionary genetic processes. Thus, plants adapted to low phosphate (P) environments exhibit only limited plasticity to take advantage of nutrient enrichment, a global phenomenon in terrestrial and aquatic environments. In the face of anthropogenic P-enrichment, low nutrient adapted resident plant species are frequently displaced by species with high morphological and genetic plasticity. However, it remains unclear whether plasticity is systemically expressed across molecular, biochemical, physiological, and morphological processes that ultimately contribute to the root and shoot phenotypes of plants. In this study, we demonstrated high plasticity in root-borne traits of sawgrass (Cladium jamaicense), the dominant plant species of the P-impoverished Everglades, and counter the idea of inflexibility in low P adapted species. However, sawgras s expressed inflexibility in processes contributing to shoot phenotypes, in contrast to cattail, which was highly plastic in shoot characteristics vii in response to P enrichment. In fact, plasticity in cattail shoots is likely a function of its growth response to P that was globally regulated by P-availability at the level of transcription. Plasticity and inflexibility in the growth of both species also diverged in their allocation of P to the chloroplast for growth in cattail versus the vacuole for P storage in sawgrass. In the Everglades, anthropogenic P-enrichment has changed the environment from a P-limited condition, where plasticity in root-borne traits of sawgrass was advantageous, to one of light-competition, where plasticity in shoot-borne traits drives competitive dominance by cattail.

Cyanobacteria are classified as alkalophiles despite their preferential uptake of the acidic form of dissolved inorganic carbon. Long term impacts of external pH on the expression of photosynthetic and structural pigments in Schizothrix calcicola were investigated as potential contributing factors to this phenomenon. More robust cell walls in S. calcicola at pH <7 are suggested by significantly greater expression of myxoxanthophylls. Direct and indirect physiological costs of altering cell walls may contribute to S. calcicola's depressed growth at acidic pH. Comparison of chlorophylls expression suggests that alkaline rather than neutral external pH is only beneficial for S. calcicola growth in absence of nutrient limitation. While the cyanobacterial biomarker ratio of chlorophylls to echinenone was stable across the pertinent pH range of 6-8, other pigment ratios in S. calcicola were affected by pH with an approximately two week lag between the change of pH and the corresponding change of pigment expression.

In recent history, C. jamaicense has been displaced by another native monocot, T. domingensis, predominantly resulting from increased phosphorous enrichment in the Everglades. This study aimed to elucidate these two species responses to low and high [Pi] in terms of allocation, photosynthate partitioning and growth. C. jamaicense growth was independent of Pi, while T. domingensis growth increased with [Pi]. Under high [Pi], allocation to younger T. domingensis shoots occurred, while C. jamaicense shoots retained more [Pi], while low [Pi] resulted in homogeneous allocation patterns for both species. Additionally, Pi deficiencies induced carbohydrate levels in older shoots of T. domingensis, while [Pi] had no effect on photosynthate partitioning patterns in C. jamaicense. ACP activity was induced by Pi deficiency in all T. domingensis shoots and increased with shoot age, while no effect was observed in C. jamaicense. Results indicate these two species differ in allocation strategies when [Pi] is altered.

In the Florida Everglades, sawgrass has been displaced by cattail, predominantly resulting from phosphate enrichment. It has been found that phosphate transporters and arbuscular mycorrhizal (AM) fungi play an important role in phosphate uptake in the plants. This study aimed to reveal the symbiosis between AM fungi and sawgrass and cattail and identify the phosphate transporters, especially AM-specific phosphate transporters in these two species. AM colonization was only found in sawgrass roots, not cattail, at low phosphate concentrations in lab and field samples by trypan blue staining. AM fungi could increase sawgrass growth and had little effect on cattail growth. Four phosphate transporters were identified in sawgrass. CjPT1, CjPT2 and CjPT3 were expressed in roots and shoots independent of AM fungi and phosphate availability, while CjPT4 appeared to be an AM regulated phosphate transporter gene and its expression was induced by AM fungi.

The study examined the past and present spatial distribution of the Florida tree snail, L. fasciatus solidus, in the Long Pine Key area of the Everglades National Park. Remote sensing and mobile GIS were used to create a GIS database of the field research results. Collection and survey-based data were used to create a current spatial distribution map of L. fasciatus solidus throughout the Long Pine Key area. The data collected during the 2006 survey were compared to a 1931 survey of the same study area conducted by Dr. William Clench of the Museum of Comparative Zoology (MCZ) of Harvard University. The data was used to determine the success of L. fasciatus solidus from 1931 to 2006, to evaluate a correlation between hammock size and the number of color forms, and to detect migration patterns of L. fasciatus solidus within the Long Pine Key area of Everglades National Park. Based on the average success rate for the seven color forms and the hammocks, the L. fasciatus solidus population in the Long Pine Key area exemplifies one of a stable community. Each color form used for the analysis had a success rate between 67% and 100%. These percentages can only be the worst case scenario based on the fact that many Liguus were not observed, and any others that would have been observed, would only increase the success rate.