Note
Charles E. Schmidt College of Science
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1979
Description
Analysis of creatine phosphokinase (CPK) isoenzymes is the
best clinical test for diagnosis of heart disease. Two new
methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies.
These new methods and the conventionally used technique
of electrophoresis are comparatively evaluated using
assayed controls and cardiac patient serum samples. Results
indicate that thiol activation and antibody inhibition are
preferred methods for CPK cardiac isoenzyme analysis because
they have lower levels of detection, fewer false negative
results, and are considerably more efficient than electrophoresis.
The new techniques also are adapted to automated spectrophotometric
instrumentation, which further contributes to
their accuracy and procedural efficiency. Thus, thiol activation
and antibody inhibition methods should provide more
sensitive and reliable CPK isoenzyme analysis for critical
supportive evidence in heart disease diagnosis and treatment
best clinical test for diagnosis of heart disease. Two new
methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies.
These new methods and the conventionally used technique
of electrophoresis are comparatively evaluated using
assayed controls and cardiac patient serum samples. Results
indicate that thiol activation and antibody inhibition are
preferred methods for CPK cardiac isoenzyme analysis because
they have lower levels of detection, fewer false negative
results, and are considerably more efficient than electrophoresis.
The new techniques also are adapted to automated spectrophotometric
instrumentation, which further contributes to
their accuracy and procedural efficiency. Thus, thiol activation
and antibody inhibition methods should provide more
sensitive and reliable CPK isoenzyme analysis for critical
supportive evidence in heart disease diagnosis and treatment
Language
Type
Form
Extent
101 p.
Subject (Topical)
Identifier
13982
Additional Information
Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1979.
FAU Electronic Theses and Dissertations Collection
Date Backup
1979
Date Text
1979
Date Issued (EDTF)
1979
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:55:28", modified_by="staff:fcllz", modification_date="2011-01-06 13:09:04"
IID
FADT13982
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Chemistry and Biochemistry
Person Preferred Name
BERNARD, DONALD JAMES.
Graduate College
Physical Description
101 p.
application/pdf
Title Plain
CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
1979
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS
Other Title Info
CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS