Model
Digital Document
Publisher
Florida Atlantic University
Description
Analysis of creatine phosphokinase (CPK) isoenzymes is the
best clinical test for diagnosis of heart disease. Two new
methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies.
These new methods and the conventionally used technique
of electrophoresis are comparatively evaluated using
assayed controls and cardiac patient serum samples. Results
indicate that thiol activation and antibody inhibition are
preferred methods for CPK cardiac isoenzyme analysis because
they have lower levels of detection, fewer false negative
results, and are considerably more efficient than electrophoresis.
The new techniques also are adapted to automated spectrophotometric
instrumentation, which further contributes to
their accuracy and procedural efficiency. Thus, thiol activation
and antibody inhibition methods should provide more
sensitive and reliable CPK isoenzyme analysis for critical
supportive evidence in heart disease diagnosis and treatment
best clinical test for diagnosis of heart disease. Two new
methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies.
These new methods and the conventionally used technique
of electrophoresis are comparatively evaluated using
assayed controls and cardiac patient serum samples. Results
indicate that thiol activation and antibody inhibition are
preferred methods for CPK cardiac isoenzyme analysis because
they have lower levels of detection, fewer false negative
results, and are considerably more efficient than electrophoresis.
The new techniques also are adapted to automated spectrophotometric
instrumentation, which further contributes to
their accuracy and procedural efficiency. Thus, thiol activation
and antibody inhibition methods should provide more
sensitive and reliable CPK isoenzyme analysis for critical
supportive evidence in heart disease diagnosis and treatment
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