Evaluation of alumina-based stationary phases for the separations of proteins and peptides by high performance liquid chromatography

Member of

FAU Theses and Dissertations

Contributors

Raghani, Anil Ratilal.

Haky, Jerome E.

Publisher

Florida Atlantic University

Date Issued

1991

Description

Alumina-based stationary phases are evaluated for the separations of proteins and peptides by reversed phase high performance liquid chromatography. Separations are compared to those obtained on a widely-used octadecylsilane (ODS) phase. The separations of peptides on alumina-based stationary phases are found to be superior while separations of proteins are inferior as compared to those found on ODS phase. The superior performance of peptide separations on alumina-based columns is attributed to lower pore size and uniquely-shaped particles of the alumina. The retentions of peptides and proteins on both alumina and silica-based stationary phases are shown to be governed by hydrophobic interaction mechanisms.

Language

English

Type

Text

Genre

Electronic Thesis or Dissertation

Form

application/pdf

Extent

117 p.

Subject (Topical)

Proteins--Separation

High performance liquid chromatography

Identifier

14719

Additional Information

Charles E. Schmidt College of Science

Thesis (M.S.)--Florida Atlantic University, 1991.

Date Backup

1991

Date Text

1991

Date Issued (EDTF)

1991

Extension

FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 03:03:19", modified_by="staff:fcllz", modification_date="2011-01-06 13:09:13"

IID

FADT14719

Issuance

monographic

Organizations

Attributed name: Florida Atlantic University

Person Preferred Name

Raghani, Anil Ratilal.
Graduate College

Physical Description

117 p.
application/pdf

PURL

http://purl.flvc.org/fcla/dt/14719