Model
Digital Document
Publisher
Florida Atlantic University
Description
Alumina-based stationary phases are evaluated for the separations of proteins and peptides by reversed phase high performance liquid chromatography. Separations are compared to those obtained on a widely-used octadecylsilane (ODS) phase. The separations of peptides on alumina-based stationary phases are found to be superior while separations of proteins are inferior as compared to those found on ODS phase. The superior performance of peptide separations on alumina-based columns is attributed to lower pore size and uniquely-shaped particles of the alumina. The retentions of peptides and proteins on both alumina and silica-based stationary phases are shown to be governed by hydrophobic interaction mechanisms.
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