Note
Thesis (M.S.)--Florida Atlantic University, 1972.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1972
Description
Cellulolytic marine isolates, Culcitalna achraspora and
Halosphaeria mediosetigera, were shaker-grown at 25 C in
artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl)
aminomethane and yeast extract, pH 7.5. Cultures were
harvested in the late linear phase where whole cell endogenous
respiration was maximal. Spectrophotometric determinations on
acetone powder extracts revealed the presence of aconitase,
NAD-linked isocitrate dehydrogenase, succinate dehydrogenase,
fumarase, malate dehydrogenase and isocitratase. Culcitalna
also showed NADP-linked isocitrate dehydrogenase activity.
Manometric examinations of acetone powder and dialyzed sonic
extracts were largely confirmatory. Oxidation of citric acid
cycle intermediates by extracts were observed with Culcitalna
for all but succinate with sonic extracts and for all but
succinate and 2-oxoglutarate in acetone powder extracts. With
Halosphaeria, neither extract could oxidize citrate or succinate,
while acetone powder extracts were unable to degrade cis-aconitate
and 2-oxoglutarate. Native mycelia were active only against
malate. Attempts to permeate cell walls with ethyl esters of
intermediates failed.
Halosphaeria mediosetigera, were shaker-grown at 25 C in
artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl)
aminomethane and yeast extract, pH 7.5. Cultures were
harvested in the late linear phase where whole cell endogenous
respiration was maximal. Spectrophotometric determinations on
acetone powder extracts revealed the presence of aconitase,
NAD-linked isocitrate dehydrogenase, succinate dehydrogenase,
fumarase, malate dehydrogenase and isocitratase. Culcitalna
also showed NADP-linked isocitrate dehydrogenase activity.
Manometric examinations of acetone powder and dialyzed sonic
extracts were largely confirmatory. Oxidation of citric acid
cycle intermediates by extracts were observed with Culcitalna
for all but succinate with sonic extracts and for all but
succinate and 2-oxoglutarate in acetone powder extracts. With
Halosphaeria, neither extract could oxidize citrate or succinate,
while acetone powder extracts were unable to degrade cis-aconitate
and 2-oxoglutarate. Native mycelia were active only against
malate. Attempts to permeate cell walls with ethyl esters of
intermediates failed.
Language
Type
Form
Extent
94 p.
Subject (Topical)
Identifier
13478
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1972.
Date Backup
1972
Date Text
1972
Date Issued (EDTF)
1972
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:08:52", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:48"
IID
FADT13478
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
VEMBU, DEVI T.
Graduate College
Physical Description
94 p.
application/pdf
Title Plain
EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI
Use and Reproduction
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Origin Information
1972
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI
Other Title Info
EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI