Model
Digital Document
Publisher
Florida Atlantic University
Description
Cellulolytic marine isolates, Culcitalna achraspora and
Halosphaeria mediosetigera, were shaker-grown at 25 C in
artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl)
aminomethane and yeast extract, pH 7.5. Cultures were
harvested in the late linear phase where whole cell endogenous
respiration was maximal. Spectrophotometric determinations on
acetone powder extracts revealed the presence of aconitase,
NAD-linked isocitrate dehydrogenase, succinate dehydrogenase,
fumarase, malate dehydrogenase and isocitratase. Culcitalna
also showed NADP-linked isocitrate dehydrogenase activity.
Manometric examinations of acetone powder and dialyzed sonic
extracts were largely confirmatory. Oxidation of citric acid
cycle intermediates by extracts were observed with Culcitalna
for all but succinate with sonic extracts and for all but
succinate and 2-oxoglutarate in acetone powder extracts. With
Halosphaeria, neither extract could oxidize citrate or succinate,
while acetone powder extracts were unable to degrade cis-aconitate
and 2-oxoglutarate. Native mycelia were active only against
malate. Attempts to permeate cell walls with ethyl esters of
intermediates failed.
Halosphaeria mediosetigera, were shaker-grown at 25 C in
artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl)
aminomethane and yeast extract, pH 7.5. Cultures were
harvested in the late linear phase where whole cell endogenous
respiration was maximal. Spectrophotometric determinations on
acetone powder extracts revealed the presence of aconitase,
NAD-linked isocitrate dehydrogenase, succinate dehydrogenase,
fumarase, malate dehydrogenase and isocitratase. Culcitalna
also showed NADP-linked isocitrate dehydrogenase activity.
Manometric examinations of acetone powder and dialyzed sonic
extracts were largely confirmatory. Oxidation of citric acid
cycle intermediates by extracts were observed with Culcitalna
for all but succinate with sonic extracts and for all but
succinate and 2-oxoglutarate in acetone powder extracts. With
Halosphaeria, neither extract could oxidize citrate or succinate,
while acetone powder extracts were unable to degrade cis-aconitate
and 2-oxoglutarate. Native mycelia were active only against
malate. Attempts to permeate cell walls with ethyl esters of
intermediates failed.
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