Note
by Paula A. Livingston.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2009
Description
Two protein tyrosine phosphatases, dual specificity phosphatase PVP and low molecular weight phosphatase WZB were purified and characterized. PVP was expressed as inclusion bodies and a suitable purification and refolding method was devised. Enzyme kinetics revealed that p-nitrophenylphosphate and (Sb(B-naphthyl phosphate were substrates with KM of 4.0mM and 8.1mM respectively. PVP showed no reactivity towards phosphoserine. Kinetic characterization of WZB showed that only pnitrophenylphosphate was a substrate with no affinity for Ç-naphthyl phosphate and phosphoserine. Optimal conditions for activity with PNPP were found at a pH of 5 with a KM of 1.1mM, kcat of 35.4s-1 and kcat/KM of 32.2s-1mM-1. Inhibition studies showed that phosphate, fluoride, and molybdate were competitive inhibitors with Ki of 3.2mM, 71.7mM, and 50.4(So(BM respectively and hydrogen peroxide abolished activity. Active site mutants of WZB Cys9Ser and Asp115Asn showed no activity.
Language
Type
Form
Extent
x, 92 p. : ill. (some col.)
Subject (Topical)
Identifier
465424465
OCLC Number
465424465
Additional Information
by Paula A. Livingston.
Thesis (M.S.)--Florida Atlantic University, 2009.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web.
Date Backup
2009
Date Text
2009
Date Issued (EDTF)
2009
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing4660", creator="creator:SPATEL", creation_date="2009-11-16 11:42:16", modified_by="super:SPATEL", modification_date="2012-04-16 12:06:59"
IID
FADT332911
Organizations
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Chemistry and Biochemistry
Person Preferred Name
Livingston, Paula A.
Graduate College
Physical Description
electronic
x, 92 p. : ill. (some col.)
Title Plain
Purification and characterization of two members of the protein tyrosine phosphatase family
Use and Reproduction
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
Boca Raton, Fla.
Florida Atlantic University
2009
Place
Boca Raton, Fla.
Title
Purification and characterization of two members of the protein tyrosine phosphatase family
Other Title Info
Purification and characterization of two members of the protein tyrosine phosphatase family
dual specificity phosphatase PVP and low molecular weight phosphatase WZB