Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2013
EDTF Date Created
2013
Description
Mitosis is the separation of duplicated chromosomes into two daughter cells
in order to create viable offspring. There are many checks in mitosis to ensure the
inherited chromosome number is correct. Sometimes, these checkpoints are
overcome and daughter cells inherit defects which can lead to cancer. One defect is
the appearance of lagging chromosomes, the result of inaccurate chromosomal
separation which leads to incorrect chromosome number termed aneuploidy.
Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism
of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in
this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the
centrosomal clustering to prevent multipolar spindles, was used in the cells.
Examining both levels of knockdown and time of exposure, we saw that the loss of
KIFC1 led to a significant increase in lagging chromosomes, indicating this protein
is critical to proper mitotic progression.
in order to create viable offspring. There are many checks in mitosis to ensure the
inherited chromosome number is correct. Sometimes, these checkpoints are
overcome and daughter cells inherit defects which can lead to cancer. One defect is
the appearance of lagging chromosomes, the result of inaccurate chromosomal
separation which leads to incorrect chromosome number termed aneuploidy.
Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism
of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in
this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the
centrosomal clustering to prevent multipolar spindles, was used in the cells.
Examining both levels of knockdown and time of exposure, we saw that the loss of
KIFC1 led to a significant increase in lagging chromosomes, indicating this protein
is critical to proper mitotic progression.
Language
Type
Genre
Form
Extent
30 p.
Identifier
FA00003505
Rights
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2013.
Date Backup
2013
Date Created Backup
2013
Date Text
2013
Date Created (EDTF)
2013
Date Issued (EDTF)
2013
Extension
FAU
IID
FA00003505
Organizations
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name
Beltran, Rosa Nathalie
Physical Description
30 p.
born digital
Title Plain
You’ve got to keep ‘em separated: characterizing lagging chromosome prevention in oral cancer cells
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Digital Origin
born digital
Origin Information
2013
2013
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
FAU Digital Library
Title
You’ve got to keep ‘em separated: characterizing lagging chromosome prevention in oral cancer cells
Other Title Info
You’ve got to keep ‘em separated: characterizing lagging chromosome prevention in oral cancer cells