Model
Digital Document
Publisher
Florida Atlantic University
Description
Mitosis is the separation of duplicated chromosomes into two daughter cells
in order to create viable offspring. There are many checks in mitosis to ensure the
inherited chromosome number is correct. Sometimes, these checkpoints are
overcome and daughter cells inherit defects which can lead to cancer. One defect is
the appearance of lagging chromosomes, the result of inaccurate chromosomal
separation which leads to incorrect chromosome number termed aneuploidy.
Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism
of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in
this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the
centrosomal clustering to prevent multipolar spindles, was used in the cells.
Examining both levels of knockdown and time of exposure, we saw that the loss of
KIFC1 led to a significant increase in lagging chromosomes, indicating this protein
is critical to proper mitotic progression.
in order to create viable offspring. There are many checks in mitosis to ensure the
inherited chromosome number is correct. Sometimes, these checkpoints are
overcome and daughter cells inherit defects which can lead to cancer. One defect is
the appearance of lagging chromosomes, the result of inaccurate chromosomal
separation which leads to incorrect chromosome number termed aneuploidy.
Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism
of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in
this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the
centrosomal clustering to prevent multipolar spindles, was used in the cells.
Examining both levels of knockdown and time of exposure, we saw that the loss of
KIFC1 led to a significant increase in lagging chromosomes, indicating this protein
is critical to proper mitotic progression.
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