Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2017
EDTF Date Created
2017
Description
The majority of HIV prevalence is found in Sub-Saharan Africa with 36.9 mil-
lion living with HIV/AIDS. The cultural implications such as patient non-compliance
or denial of available routine medical care can potentially cause limitations on the ef-
fectiveness of detecting such virulent pathogens and manage chronic disease. The lack
of access to healthcare and further socioeconomic impacts hinder the ability to ade-
quately diagnose and treat infection in resource-limited settings. Intervention through
diagnosis and treatment helps prevent the spread of transmission, where pre-exposure
prophylaxis or active disease prevention measures are not readily available. The cur-
rent gold standard for HIV detection is by molecular detection; Reverse-Transcription
Polymerase Chain Reaction is widely used that employs cycles of temperature condi-
tions that require a thermal cycling platform and typically laboratory space for RNA
extraction separate from RT-PCR space required. Serological detection can be ad-
vantageous for surveillance and screening, Lateral Flow Assays and Enzyme-Linked
Immunosorbent Assay (ELISA) can detect a viral protein (antigen) or antibodies.
The ELISA can require at least 12 hours of assay preparation and takes a diagnostic
laboratory many resources to run. There is need to develop Point-of-Care (POC) testing that can potentially be used for decentralized testing that can leverage ex-
isting technologies such as smart phone capability and routine medical or diagnostic
tests with cutting edge applications leveraging micro
uidics, nanotechnology and in-
tegrated circuit design. Such technologies allow for automated, rapid turnaround
and cost-e ective diagnosis of HIV, where these assays could potentially be read-
ily deployed. It is such technology that can potentially change the way diagnostics
are performed, as POC technology can be rapidly disseminated, enable decentralized
testing and, is user-friendly. A novel smart phone-enabled automated magnetic bead-
based platform was developed for a micro
uidic ELISA for HIV-1 detection at the
POC to meet this demand.
lion living with HIV/AIDS. The cultural implications such as patient non-compliance
or denial of available routine medical care can potentially cause limitations on the ef-
fectiveness of detecting such virulent pathogens and manage chronic disease. The lack
of access to healthcare and further socioeconomic impacts hinder the ability to ade-
quately diagnose and treat infection in resource-limited settings. Intervention through
diagnosis and treatment helps prevent the spread of transmission, where pre-exposure
prophylaxis or active disease prevention measures are not readily available. The cur-
rent gold standard for HIV detection is by molecular detection; Reverse-Transcription
Polymerase Chain Reaction is widely used that employs cycles of temperature condi-
tions that require a thermal cycling platform and typically laboratory space for RNA
extraction separate from RT-PCR space required. Serological detection can be ad-
vantageous for surveillance and screening, Lateral Flow Assays and Enzyme-Linked
Immunosorbent Assay (ELISA) can detect a viral protein (antigen) or antibodies.
The ELISA can require at least 12 hours of assay preparation and takes a diagnostic
laboratory many resources to run. There is need to develop Point-of-Care (POC) testing that can potentially be used for decentralized testing that can leverage ex-
isting technologies such as smart phone capability and routine medical or diagnostic
tests with cutting edge applications leveraging micro
uidics, nanotechnology and in-
tegrated circuit design. Such technologies allow for automated, rapid turnaround
and cost-e ective diagnosis of HIV, where these assays could potentially be read-
ily deployed. It is such technology that can potentially change the way diagnostics
are performed, as POC technology can be rapidly disseminated, enable decentralized
testing and, is user-friendly. A novel smart phone-enabled automated magnetic bead-
based platform was developed for a micro
uidic ELISA for HIV-1 detection at the
POC to meet this demand.
Language
Type
Form
Extent
69 p.
Subject (Topical)
Identifier
FA00005945
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2017.
FAU Electronic Theses and Dissertations Collection
Date Backup
2017
Date Created Backup
2017
Date Text
2017
Date Created (EDTF)
2017
Date Issued (EDTF)
2017
Extension
FAU
FAU
IID
FA00005945
Organizations
Attributed name: Florida Atlantic University
Attributed name: College of Engineering and Computer Science
Person Preferred Name
Coarsey, Chad Thomas
author
Graduate College
Physical Description
application/pdf
69 p.
Title Plain
Development of Smart Phone-based Automated Microfluidic-ELISA For Human Immunodefciency Virus 1
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2017
2017
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Development of Smart Phone-based Automated Microfluidic-ELISA For Human Immunodefciency Virus 1
Other Title Info
Development of Smart Phone-based Automated Microfluidic-ELISA For Human Immunodefciency Virus 1