Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2018
EDTF Date Created
2018
Description
In Drosophila melanogaster, the GFS is synaptically coupled to the
Tergotrochanteral motoneurons; these neurons form a signaling pathway from the brain to
the jump muscles (Thomas and Wyman, 1983). Part of this signaling is done through gap
junctions, and placement of these gap junctions was partially shown to be regulated by the
binding of Netrin, a class of guidance molecule (Orr et al., 2014). In the present study we
investigate the role of Netrin's receptor Frazzled in the placement of gap junctions in
Drosophila at: 1) Presynaptic neurons (Giant Fibers [GF]), 2) Postsynaptic neurons
(Tergotrochanteral motoneurons [TTMn]), and 3) Presynaptic + Postsynaptic neurons
simultaneously. Effects of Frazzled were tested using Frazzled RNAi and a combination
of electrophysiological recordings and imaging of the GF-TTMn synapse. The results from
this study show that presynaptic and postsynaptic knockdown of Frazzled delayed
muscular responses and altered the anatomy of both the GF's and TTMn's.
Tergotrochanteral motoneurons; these neurons form a signaling pathway from the brain to
the jump muscles (Thomas and Wyman, 1983). Part of this signaling is done through gap
junctions, and placement of these gap junctions was partially shown to be regulated by the
binding of Netrin, a class of guidance molecule (Orr et al., 2014). In the present study we
investigate the role of Netrin's receptor Frazzled in the placement of gap junctions in
Drosophila at: 1) Presynaptic neurons (Giant Fibers [GF]), 2) Postsynaptic neurons
(Tergotrochanteral motoneurons [TTMn]), and 3) Presynaptic + Postsynaptic neurons
simultaneously. Effects of Frazzled were tested using Frazzled RNAi and a combination
of electrophysiological recordings and imaging of the GF-TTMn synapse. The results from
this study show that presynaptic and postsynaptic knockdown of Frazzled delayed
muscular responses and altered the anatomy of both the GF's and TTMn's.
Language
Type
Form
Extent
63 p.
Subject (Topical)
Identifier
FA00013085
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2018.
FAU Electronic Theses and Dissertations Collection
Date Backup
2018
Date Created Backup
2018
Date Text
2018
Date Created (EDTF)
2018
Date Issued (EDTF)
2018
Extension
FAU
IID
FA00013085
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Biological Sciences
Person Preferred Name
Lopez, Juan
author
Graduate College
Physical Description
application/pdf
63 p.
Title Plain
Frazzled’s Role in Synapse Formation at a Drosophila Giant Synapse
Use and Reproduction
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Origin Information
2018
2018
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Frazzled’s Role in Synapse Formation at a Drosophila Giant Synapse
Other Title Info
Frazzled’s Role in Synapse Formation at a Drosophila Giant Synapse