Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2007
EDTF Date Created
2007
Description
tmRNA is a small stable RNA present in Eubacteria. Through a mechanism called
trans-translation, tmRNA mediates ribosome rescue and quality control of proteins and
mRNA. In this study, the Escherichia coli (E. coli) mutant lacking tmRNA was
demonstrated hypersensitive to oxidative stress. The role of tmRNA-mediated
surveillance mechanism in protecting E. coli cell under oxidative stress condition was
examined. The tmRNA-mediated tagged protein levels were elevated in cells under
oxidative stress condition, demonstrating the enhanced need for tmRNA under such
condition. Our results suggest that mRNA damage by oxidative stress may cause reduced
cell viability, and that tmRNA is required to rescue cells under such condition.
Furthermore, our observations showed that tmRNA is required for the optimal growth of
E. coli under normal aeration but not under anaerobic condition, suggesting that oxidation
ofmRNA is the major reason for requirement oftmRNA during normal aeration.
trans-translation, tmRNA mediates ribosome rescue and quality control of proteins and
mRNA. In this study, the Escherichia coli (E. coli) mutant lacking tmRNA was
demonstrated hypersensitive to oxidative stress. The role of tmRNA-mediated
surveillance mechanism in protecting E. coli cell under oxidative stress condition was
examined. The tmRNA-mediated tagged protein levels were elevated in cells under
oxidative stress condition, demonstrating the enhanced need for tmRNA under such
condition. Our results suggest that mRNA damage by oxidative stress may cause reduced
cell viability, and that tmRNA is required to rescue cells under such condition.
Furthermore, our observations showed that tmRNA is required for the optimal growth of
E. coli under normal aeration but not under anaerobic condition, suggesting that oxidation
ofmRNA is the major reason for requirement oftmRNA during normal aeration.
Language
Type
Form
Extent
113 p.
Subject (Topical)
Identifier
FA00000782
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2007.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
2007
Date Created Backup
2007
Date Text
2007
Date Created (EDTF)
2007
Date Issued (EDTF)
2007
Extension
FAU
IID
FA00000782
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Medicine
Attributed name: Department of Biomedical Science
Person Preferred Name
Kollipara, Gayatri
Graduate College
Physical Description
application/pdf
113 p.
Title Plain
Study on the Role oftmRNA in Protecting Escherichia coli Cell Under Oxidative stress.
Use and Reproduction
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Origin Information
2007
2007
Florida Atlantic University
Boca Raton, FL
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, FL
Sub Location
Digital Library
Title
Study on the Role oftmRNA in Protecting Escherichia coli Cell Under Oxidative stress.
Other Title Info
Study on the Role oftmRNA in Protecting Escherichia coli Cell Under Oxidative stress.