Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2017
EDTF Date Created
2017
Description
Extracellular stimuli may influence the M1/M2 phenotypic polarization of
macrophages. We examined M1/M2 biomarkers, phagocytic activity, and tumoricidal
activity in RAW 264.7 mouse macrophages. Macrophages were treated with conditioned
media (CM) from 4T1 breast cancer cells, curcumin, 22-oxacalcitriol, LPS, or a
combination of the previously listed. Arginase activity, a M2 phenotypic biomarker, was
upregulated by the treatment of macrophages with conditioned media. Curcumin, 22-
oxacalcitriol, and LPS partially inhibited RAW 264.7 arginase activity in the presence of
4T1 breast cancer media. 22-oxacalcitriol increased the phagocytic ability of RAW 264.7
macrophages in the presence of M2 polarizing substances produced by the 4T1 breast
cancer cells. Also, LPS increased RAW 264.7 phagocytic ability in the presence of 4T1
breast cancer CM. This study looked at the potential substances that would possibly reverse the M2 tumor promoting macrophage phenotype seen in the breast cancer tumor
environment.
macrophages. We examined M1/M2 biomarkers, phagocytic activity, and tumoricidal
activity in RAW 264.7 mouse macrophages. Macrophages were treated with conditioned
media (CM) from 4T1 breast cancer cells, curcumin, 22-oxacalcitriol, LPS, or a
combination of the previously listed. Arginase activity, a M2 phenotypic biomarker, was
upregulated by the treatment of macrophages with conditioned media. Curcumin, 22-
oxacalcitriol, and LPS partially inhibited RAW 264.7 arginase activity in the presence of
4T1 breast cancer media. 22-oxacalcitriol increased the phagocytic ability of RAW 264.7
macrophages in the presence of M2 polarizing substances produced by the 4T1 breast
cancer cells. Also, LPS increased RAW 264.7 phagocytic ability in the presence of 4T1
breast cancer CM. This study looked at the potential substances that would possibly reverse the M2 tumor promoting macrophage phenotype seen in the breast cancer tumor
environment.
Language
Type
Form
Extent
41 p.
Subject (Topical)
Identifier
FA00004867
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2017.
FAU Electronic Theses and Dissertations Collection
Date Backup
2017
Date Created Backup
2017
Date Text
2017
Date Created (EDTF)
2017
Date Issued (EDTF)
2017
Extension
FAU
IID
FA00004867
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Biological Sciences
Person Preferred Name
Liddle, Genevieve M.
author
Graduate College
Physical Description
application/pdf
41 p.
Title Plain
A Study on Reversing the Immunosuppressive Phenotype of Tumor Associated Macrophages
Use and Reproduction
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Origin Information
2017
2017
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
A Study on Reversing the Immunosuppressive Phenotype of Tumor Associated Macrophages
Other Title Info
A Study on Reversing the Immunosuppressive Phenotype of Tumor Associated Macrophages