Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2016
EDTF Date Created
2016
Description
The purpose of this research was to analyze the regulatory
pattern of the PUN promoter in the expression
of a marker gene, β-glucoronidase (GUS), within regenerated
tobacco plants. The genes for neomycin
phosphotransferase (NPT II) and GUS were included
in the coding region of the Ti plasmid construct. The
NPTII gene drove antibiotic resistance and was used
to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical
staining and genetic assays rendered putative
transgenic lines that were cultivated for further
assessment of progeny. First generation histochemical
analysis of 14-day tissue formation resulted in no
levels of expression for the GUS gene, which demonstrated
that the flower-specific PUN promoter was
not active in the leaf tissue. Further testing of gene
activity throughout all stages of tissue formation for
the first generation lines is required in order to assess
regulatory pattern of the PUN promoter.
pattern of the PUN promoter in the expression
of a marker gene, β-glucoronidase (GUS), within regenerated
tobacco plants. The genes for neomycin
phosphotransferase (NPT II) and GUS were included
in the coding region of the Ti plasmid construct. The
NPTII gene drove antibiotic resistance and was used
to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical
staining and genetic assays rendered putative
transgenic lines that were cultivated for further
assessment of progeny. First generation histochemical
analysis of 14-day tissue formation resulted in no
levels of expression for the GUS gene, which demonstrated
that the flower-specific PUN promoter was
not active in the leaf tissue. Further testing of gene
activity throughout all stages of tissue formation for
the first generation lines is required in order to assess
regulatory pattern of the PUN promoter.
Language
Type
Genre
Form
Extent
1 p.
Subject (Topical)
Identifier
FA00005606
Date Backup
2016
Date Created Backup
2016
Date Text
2016
Date Created (EDTF)
2016
Date Issued (EDTF)
2016
Extension
FAU
IID
FA00005606
Organizations
Attributed name: Office of Undergraduate Research and Inquiry
Person Preferred Name
Velez, Stephanie
Physical Description
application/pdf
1 p.
Title Plain
Regulatory Pattern of PUN Promoter for Gene Expression
Origin Information
2016
2016
Florida Atlantic University
Boca Raton, Florida
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Florida
Sub Location
Digital Library
Title
Regulatory Pattern of PUN Promoter for Gene Expression
Other Title Info
Regulatory Pattern of PUN Promoter for Gene Expression