Model
Digital Document
Publisher
Florida Atlantic University
Description
The purpose of this research was to analyze the regulatory
pattern of the PUN promoter in the expression
of a marker gene, β-glucoronidase (GUS), within regenerated
tobacco plants. The genes for neomycin
phosphotransferase (NPT II) and GUS were included
in the coding region of the Ti plasmid construct. The
NPTII gene drove antibiotic resistance and was used
to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical
staining and genetic assays rendered putative
transgenic lines that were cultivated for further
assessment of progeny. First generation histochemical
analysis of 14-day tissue formation resulted in no
levels of expression for the GUS gene, which demonstrated
that the flower-specific PUN promoter was
not active in the leaf tissue. Further testing of gene
activity throughout all stages of tissue formation for
the first generation lines is required in order to assess
regulatory pattern of the PUN promoter.
pattern of the PUN promoter in the expression
of a marker gene, β-glucoronidase (GUS), within regenerated
tobacco plants. The genes for neomycin
phosphotransferase (NPT II) and GUS were included
in the coding region of the Ti plasmid construct. The
NPTII gene drove antibiotic resistance and was used
to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical
staining and genetic assays rendered putative
transgenic lines that were cultivated for further
assessment of progeny. First generation histochemical
analysis of 14-day tissue formation resulted in no
levels of expression for the GUS gene, which demonstrated
that the flower-specific PUN promoter was
not active in the leaf tissue. Further testing of gene
activity throughout all stages of tissue formation for
the first generation lines is required in order to assess
regulatory pattern of the PUN promoter.
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