Cell cycle analysis of primary sponge cell cultures

Member of
HBOI Faculty Contributions
Contributors
Schippers, K. J.
Martens, D. E.,
Pomponi, Shirley A.
Wijffels, R. H.
Publisher
Springer
Date Issued
2011
Note

Proliferation of sponge cells is generally measured via cell counts or viability assays. However, more insight into the proliferative state of a sponge cell population can be obtained from the distribution of the cells over the different phases of the cell cycle. Cell cycle distribution of sponge cells was measured via flow cytometry after staining the DNA with propidium iodide. The five sponges studied in this paper all showed a large fraction of cells in G1/G0 compared to G2/M and S, indicating that cells were not actively dividing.

Language
English
Type
Text
Genre
article
Extent
11 p.
Subject (Topical)
Sponges--Cytology
Cell cycle
Cell proliferation
Flow cytometry
Identifier
3318911
Additional Information
Proliferation of sponge cells is generally measured via cell counts or viability assays. However, more insight into the proliferative state of a sponge cell population can be obtained from the distribution of the cells over the different phases of the cell cycle. Cell cycle distribution of sponge cells was measured via flow cytometry after staining the DNA with propidium iodide. The five sponges studied in this paper all showed a large fraction of cells in G1/G0 compared to G2/M and S, indicating that cells were not actively dividing.
This manuscript is an author version with the final publication available at http://www.springerlink.com and may be cited as: Schippers, K. J., Martens, D. E., Pomponi, S. A., & Wijffels, R. H. (2011). Cell cycle analysis of primary sponge cell cultures. In Vitro Cellular & Developmental Biology - Animal, 47(4), 302-311. doi:10.1007/s11626-011-9391-x
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1831.
Date Backup
2011
Date Text
2011
DOI
10.1007/s11626-011-9391-x
Date Issued (EDTF)
2011
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing11258", creator="creator:NBURWICK", creation_date="2011-10-26 15:50:22", modified_by="super:FAUDIG", modification_date="2014-02-12 10:39:19"

IID
FADT3318911
Issuance
single unit
Person Preferred Name

Schippers, K. J.

creator

Physical Description

pdf
11 p.
PURL
http://purl.flvc.org/FCLA/DT/3318911
Title Plain
Cell cycle analysis of primary sponge cell cultures
URL
http://purl.flvc.org/FCLA/DT/3318911
Origin Information

Springer
2011
single unit
Title
Cell cycle analysis of primary sponge cell cultures
Other Title Info

Cell cycle analysis of primary sponge cell cultures