Note
Charles E. Schmidt College of Science
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2007
EDTF Date Created
2007
Description
The composition of marine bacterial communities from South Florida beaches
were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured
populations, community genomic DNA was extracted directly from sand and
seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct
extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in
Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70),
the populations cluster into several unknown clads with early divergence, indicating the
presence of novel well established bacterial groups. Members of phylum Bacteroidetes,
Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in
culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach.
Taxonomic representation indicates marine water as more diverse compared to dry sand
and wet sand. A combination of phylogenetic markers will be needed to define the
immense diversity of this niche.
were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured
populations, community genomic DNA was extracted directly from sand and
seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct
extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in
Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70),
the populations cluster into several unknown clads with early divergence, indicating the
presence of novel well established bacterial groups. Members of phylum Bacteroidetes,
Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in
culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach.
Taxonomic representation indicates marine water as more diverse compared to dry sand
and wet sand. A combination of phylogenetic markers will be needed to define the
immense diversity of this niche.
Language
Type
Form
Extent
189 p.
Subject (Topical)
Identifier
FA00000809
Additional Information
Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 2007.
Date Backup
2007
Date Created Backup
2007
Date Text
2007
Date Created (EDTF)
2007
Date Issued (EDTF)
2007
Extension
FAU
IID
FA00000809
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Ponukumati, Sushma
Graduate College
Physical Description
application/pdf
189 p.
Title Plain
Elucidation of Microbial Communities From South Florida Beaches Using 16S rRNA Sequence Analysis
Use and Reproduction
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Origin Information
2007
2007
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Elucidation of Microbial Communities From South Florida Beaches Using 16S rRNA Sequence Analysis
Other Title Info
Elucidation of Microbial Communities From South Florida Beaches Using 16S rRNA Sequence Analysis