thesis

Aconitase is an important enzyme in the citric Acid Cycle, is needed for maintenance of mitochondrial DNA, is a key regulator of iron in the cell, and is very sensitive to oxidative stress. We have isolatd the yeast ACO1 gene, which codes for aconitase, and randomly mutated it to create a mutant library of cells each expressing a different version of ACO1. We will select for oxidative stress resistant aconitase in S. cerevisiae by subjecting strains to successive rounds of heat shock and competitive growth against other mutants. The "winner" of this competition will then be analyzed for which version of aconitase it is expressing, which may lead to increased longevity.

This thesis considers the role of two rhetoricians, Petruchio from William Shakespeare's The Taming of the Shrew and Captain Ahab from Herman Melville's Moby-DIck, and analyzes their use of verbal performances. BOth Petruchio and Captain Ahab use pathologically manipulative rhetoric to manipulate others emotionally. Through this manipulation, they attempt to gain control, power, and authority. While both Petrucio and Ahab appear to succeed in manipulating others, they actually fail. Petruchio attempts to manipulate Katherine, but fails to attain mental submission from her, and Captain Ahab attempts to manipulate the crew to pursue hunting the whale, but as the narrative progresses, the crew becomes too disillusioned with the hunt to be persuaded by Ahab's rhetoric. In conclusion, both Petruchio and Captain Ahab are unable to sustain rhetorical control, and they both fall into demagoguery, therby suggesting that while they are alike as rhetoricians, they both fail similarly as rhetoricians.

The surge in granting equal rights to gays and lesbians in the United States is remarkable. Yet with this surge comes a conflict : the civil rights of gays and lesbians against the rights of religious individuals, predominantly Christians, refusing to tolerate a behavior they think immoral. My thesis focuses on two hypothetical situations : a county clerk refusing to issue a marriage license to an engaged lesbian couple and an inn owner refusing a night's stay to a gay couple. In both cases, the clerk and inn owner refuse service for religious reasons. Normatively, I argue that we must move beyond a framework of toleration to a system of equal respect and understanding of our fellow human beings. Legally, I argue that the rights of religious expression and exercise should not trump the civil rights of gays and lesbians in the public sphere.

The works of Romantic poet William Wordsworth are generally regarded as idealistic nature poems. However, Wordsworth was writing in a turbulent era, between the French Revolution and the Industrial Revolution. Contrary to conventional labels, Wordsworth's prose and poetry of the late eighteenth and early nineteenth centuries strongly critiques social and economic affairs, similar to the ways Thomas Malthus comments on the same subjects. In 1798, political and economic theorist Thomas Robert Malthus published his infamous Essay on the Principle of Population, in which he devotes considerable thought to the subjects of poverty and England's Old Poor Law system. This thesis explores the connections between Wordsworth and Malthus, establishing Wordsworth as an amateur political economic theorist, who was concerned with the contemporary treatment of poverty and the morals of the legislators of the Poor Laws. I further claim that Wordsworth was a parable-poet, who sought to provide moral guidance regarding poor relief through affective poetry.

In my thesis work, I attempted to construct a plasmid that would allow stable integration of genes into the Saccharomyces cerevisiae yeast genome under the control of the repressible TetO promoter. The yeast ACO1 gene was cloned under the control of the TetO operator and the tTA transactivator. This construct was inserted into yeast cells in order to observe the effects of aconitase overexpression on aging. Unfortunately, the transformed cells appeared incapable of aconitase expression as determined by glutamic acid auxptrophy, a phenotype of aconitase mutants. We have sequenced the pIT1ACO1 plasmid and have found many abnormalities in the promoter region. If the plasmid can be made to function as intended, the resulting yeast strain can be used in the future to determine if aconitase plays an important role in cellular aging.

Tumor cells are characterized by an increase in genomic instability, brought about by both chromosomal rearrangement and chromosomal instability. Both of these broad changes can be induced by exposure to carcinogens. During mitosis, cells can exhibit early and late lagging chromosomes, multipolar spindles or anaphase bridges, all of which contribute to genomic rearrantement. We have studied the link between exposure to carcinogen and prevalence of mitotic defect in both chromosomally stable and unstable cell lines as well as ecamined the restorative effects of antioxidants in preventing mitotic defects. We have exposed MES-SA uterine cancer cells to vinyl chloride followed by exposure to an antioxidant : ascorbic acid, B-carotene, or lycopene. Treated cells were then scored for the prevalence of mitotic defects within the population and compared to controls. We have also investigated whether pre-treatment with the antioxidants will weaken the effects of carcinogen exposure in these cell lines.

This paper examines early modern climatology in order to analyze a correlation between weather, witchcraft, and their combined effects on Shakespeare's Macbeth. Contemporary climactic patterns including hailstorms, tempests, and other unusual phenomena drastically affected not only England but much of Europe. Religious and scientific texts of the period examined links between weather phenomena and witchcraft, suggesting that the weather afflictions were caused by witchcraft. Shakespeare incorporates this suggestion into Macbeth, as well, but takes the connection even further ; the witches in the play not only use their weather magic to afflict Scotland but also control the events of the play.

A crucial aspect of captive animal care is enrichment, ranging from simple additions of flora to complicated foraging puzzles. Enrichments are vital in creating an environment for animals that is similar to their wild habitat and eliciting natural behaviors. Enrichment is also used to inhibit unnatural or harmful behaviors, like pacing. Commonly, enrichment techniques are not scientifically tested, and the universality of enrichments is rarely scrutinized. This study explores the universality of a "Snak'n'Trim", a hollow ball 10 inches in diameter with a 1 inch opening for food items to be inserted into the ball. Four bears, (two male, two female) were observed for ten weeks, five in the summer and five in the winter. Daily observations were made during baseline and enrichment trials. Behavior frequency was calculated and results indicate a significant (p<.01) difference between baseline and enrichment trials, providing evidence for the efficacy of the Snak'n'Trim.

The felony-murder rule declares that if a death occurs during the commission of a felony, all persons involved in the felony will be held culpable for the death. The rule makes every person equally culpable for the death, regardless of his or her mental state and degree of involvement during the crime. Drawing on theories of punishment and scholarship on culpability, I argue that the felony-murder rule needs to be modified. Each person involved in the crime should not be held accountable for the actions of another, but should only be held responsible and culpable for their own intended actions.

This study assesses the philosophical underpinnings of endangered species protections focused on gopher tortoises in Florida. From 1993-2007, gopher tortoises (Gopherus polyphemus) were listed in Florida as a species of special concern. Land developers wanting to build on their habitats could apply for an incidental take permit, paying a fee that allowed tortoises to be 'entombed' in their burrows to die a slow death. In 2007, when gopher tortoise status was up-losted to 'threatened', a new policy ended the incidental take permit program. This study compares policy guidelines of the 1991 and 2007 gopher tortoise management plans and explores cases that deal with incidental take controversies. I will apply perspectives from environmental philosophy to my analysis to understand how policies attempt to balance economic goals with a mandate to protect species in peril.