Model
Digital Document
Publisher
Florida Atlantic University
Description
The molar and weight ratios of the photosynthetic pigments of ten algal species were
analyzed in an attempt to determine if knowledge of the light field could be used to
give better ratios for estimating the chlorophyll a contributed from each algal division
to total chlorophyll a or biomass. High performance liquid chromatography (HPLC)
is one of the tools used in pigment-based chemotaxonomy for estimating the biomass
of algal communities by determining the concentrations of the various chlorophylls
and carotenoids. Stable ratios of chlorophyll a: marker pigment are used as
multiplying factors for calculating the amount of chlorophyll a contributed from each
major algal group to total chlorophyll a. Stable chlorophyll a : marker pigment ratios
are not always easy to determine, so light field was investigated as a factor for
determining consistently reliable ratios. This data will serve to determine new
chlorophyll a: marker pigment ratios, as well as to verify or suggest changes to the
existing ratios that are currently being used by our research group. An unknown
pigment was isolated from Scytonema sp. grown in higher light fields and the
ecological significance of this pigment was assessed.
analyzed in an attempt to determine if knowledge of the light field could be used to
give better ratios for estimating the chlorophyll a contributed from each algal division
to total chlorophyll a or biomass. High performance liquid chromatography (HPLC)
is one of the tools used in pigment-based chemotaxonomy for estimating the biomass
of algal communities by determining the concentrations of the various chlorophylls
and carotenoids. Stable ratios of chlorophyll a: marker pigment are used as
multiplying factors for calculating the amount of chlorophyll a contributed from each
major algal group to total chlorophyll a. Stable chlorophyll a : marker pigment ratios
are not always easy to determine, so light field was investigated as a factor for
determining consistently reliable ratios. This data will serve to determine new
chlorophyll a: marker pigment ratios, as well as to verify or suggest changes to the
existing ratios that are currently being used by our research group. An unknown
pigment was isolated from Scytonema sp. grown in higher light fields and the
ecological significance of this pigment was assessed.
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