Model
Digital Document
Publisher
Rosenstiel School of Marine and Atmospheric Science, University of Miami
Member of
Coral reef ecology
Model
Digital Document
Publisher
Rosenstiel School of Marine and Atmospheric Science, University of Miami
Member of
Model
Digital Document
Publisher
Springer
Member of
Model
Digital Document
Publisher
Springer-Verlag.
Member of
Model
Digital Document
Publisher
Elsevier B.V.
Member of
Model
Digital Document
Publisher
Elsevier Ltd.
Member of
Model
Digital Document
Publisher
Gulf and Caribbean Fisheries Institute
Member of
Model
Digital Document
Publisher
Elsevier B.V.
Member of
Model
Digital Document
Publisher
Elsevier B.V.
Member of
Model
Digital Document
Publisher
Florida Atlantic University
Description
The gorgonian Briareum asbestinum is widely studied because it possesses highly oxygenated novel structures, many of which exhibit useful biological activities. Recently, two new briarane diterpenoids, briareolate esters J and K, together with two known briareolate esters have been isolated from a specimen of Briareum asbestinum collected off the coast of Boca Raton, Florida. The method used was a 96-well plate real-time cell electronic sensing (RT-CES) system to discover compounds that impact human embryonic stem cell growth. The compounds were isolated using reversed phase polystyrene divinylbenzene chromatographic support HP20ss followed by normal phased HPLC using a luna silica column. The structures of the compounds were established though the interpretation of spectroscopic data. Activity testing was conducted against hESCs (BG02) with briareolate ester J showing no inhibition activity and briareolate ester K showing mild activity with an EC50 value of 25 (So(BM. These results confirm that the exact confirmation and existence of the (E,Z)-dienone is related to the activity that was observed with the previously isolated briareolate esters L and M.
Member of