Methodology

This study examines whether obesity affects the accuracy of estimating age-at-death as measured by the age-related changes of the pubic symphysis and auricular surfaces. I scored the hip bones of 119 adults of normal body mass and 126 obese adults (total n = 254) using the SucheyBrooks (1990) method for the pubic symphysis and the Buckberry and Chamberlain (2002) method for the auricular surface. Compared to normal weight individuals, obese individuals exhibited greater inaccuracy in age-at-death estimates when aged from the auricular surface, but not the pubic symphysis. However, age was estimated with less precision in obese individuals using both methods. Obese males are more likely to be aged inaccurately than obese females. The pubic symphysis method may be the preferred method when estimating age in obese individuals, especially males, but forensic anthropologists should use caution when assessing age-at-death in obese adults using either method.

Tumor cells interact with basement membrane collagen at the site of extravasation through distinct cellular receptors, including the α2β1 and α3β1integrins. These receptors are known to be differentially expressed in metastatic tumors, relative to the normal cells, depending on tumor type and stage of progression. The binding sites within type IV collagen for the α2β1 andα3β1 integrins have been identified. Since both of the integinspecific sequences possess at least one glycosylated Hyl residue, we questioned whether glycosylation could modulate integrin binding. Triple-helical peptides with and without Lys substituted by glycosylated Hyl for Lys543 and Lys540 from the human a1(IV)531-543 gene sequence (α3β integrin-specific) and Lys393 from the human a1(IV)382-393 gene sequence (α2β1 integrin-specific) were synthesized and utilized in the present study.

Microarray expression data which contains the expression levels of a large number of simultaneously observed genes have been used in many scientific research and clinical studies. Due to its high dimensionalities, selecting a small number of genes has shown to be beneficial for many tasks such as building prediction models from the microarray expression data or gene regulatory network discovery. Traditional gene selection methods, however, fail to take the class distribution into the selection process. In biomedical science, it is very common to have microarray expression data which is severely biased with one class of examples (e.g., diseased samples) significantly less than other classes (e.g., normal samples). These sample sets with biased distributions require special attention from researchers for identification of genes responsible for a particular disease. In this thesis, we propose three filtering techniques, Higher Weight ReliefF, ReliefF with Differential Minority Repeat and ReliefF with Balanced Minority Repeat to identify genes responsible for fatal diseases from biased microarray expression data. Our solutions are evaluated on five well-known microarray datasets, Colon, Central Nervous System, DLBCL Tumor, Lymphoma and ECML Pancreas. Experimental comparisons with the traditional ReliefF filtering method demonstrate the effectiveness of the proposed methods in selecting informative genes from microarray expression data with biased sample distributions.

This study explored the types and uses of informal, on-the-job learning occurring among American Speech-Language-Hearing (ASHA)-certified Speech-Language Pathologists (SLPs) working in medical settings as part of a rehabilitation team. In addressing this purpose, a qualitative, phenomenological approach to research methodology was used. Participants were selected through a process of purposeful sampling, each meeting the criteria of having earned the Certificate of Clinical Competence in Speech-Language Pathology from ASHA and working in a medical setting as part of a rehabilitation team. Twenty-four SLPs from varying demographic profiles took part in one of three online data collection methods. Nine participants completed a computer-mediated interview, 11 participants completed an online journal entry and 4 participants completed a virtual focus group method. All data collection methods were accomplished via internet technologies. Data analysis was performed through a process of thematic analysis and resulted in four significant findings. First, both internal and external forces triggered SLPs to learn informally in the medical workplace. Also, SLPs went through an individually-determined, goal-directed process when learning informally in the medical workplace. SLPs used a combination of collaboration, workplace experience and research to learn informally in the medical workplace. And finally, SLPs employed informal learning activities for the purpose of meeting personal, patient and organizational needs.

Locus of an author poses questions of intentionality, how intention is discovered, expressed, hidden, revealed and interpreted. The purpose of the study is to find and apply productive interdisciplinary concepts in intentionality detection, decoding and evaluation in fictional texts. The investigation integrates traditions in literature, linguistics, cognitive science and creative writing, posing a pragmatics of intent that complements and complicates precepts in reader reception-based constructivism. Basic to a vision of pragmatic strategies: 1) situating effect and affect in an embodied mind; 2) acknowledging mutual and/or oppositional intentionalities which an embodied author and embodied reader bring to the process of fictional communication; 3) accepting language as communication that requires cognitive translation of consensually-agreed upon symbols into private representations in an embodied mind; 4) assuming that an author's fictionalizing consciousness is more discernible w hen it is navigating tensions of selection, proportion, intervention and perspective. Perceptual and close reading of J.M. Coetzee's Foe yields descriptive problematics. Analytical readings in a neglected byway of I.A. Richards' New Criticism provide pragmatic cues for detecting and evaluating intentionalities in prose. Three cross-disciplinary strategies emerge to enhance perceptual and close readings of fictional texts: 1) awareness of priming effects in form and content; 2) identification of markedness patterns; and 3) perception of tensible connections in prosaic language and artistic devices.

Public administration addresses issues that competing and aligning groups determine to be meaningful enough to address. However, there seems to be no shared universally objective ways of remedying anything. Everything is up for argument. Additionally, attempting to solve one set of problems often creates other connected problems and/or unintended consequences. So, public work ever [sic] never ends. This dissertation's purpose was to contribute a new theoretical understanding of the experience and practice of public administration. Its research addressed if and how a grounded existential theoretical framework could emerge that would help practitioners and scholars understand and describe public administrative efforts and experiences. Currently, there is no existential theory of public administration. This dissertation sought to initiate work in that direction. This dissertation employed a grounded theory methodology to collect information from Senior Executive Service (SES) members, to analyze the information for emerging concepts and theoretical relevance through constant comparison, and to discover/construct a theoretical framework for understanding public administrative efforts and experiences. "The grounded theory approach is a general methodology of analysis linked with data collection that uses a systematically applied set of methods to generate an inductive theory about a substantive area" (Glaser, 1992, p. 16).

Sorites dominicensis is a common Foraminifera living on Thalassia testudinum seagrass blades in the Indian River Lagoon. This locality is unique because epiphytic diatoms, characteristic of Caribbean and temperate environments, are expected to make up the biofilm community. Diatoms compose a large part of the Foraminiferan diet. It is important to understand the cause and effect relationship of population variation between S. dominicensis and its preferred food source, since both are valuable bioindicators. We examined the selective feeding of S. dominicensis by first, identifying the diatom assemblage. Second, we utilized scanning electron microscopy (SEM) in order to identifying the remains of diatoms entrapped in pseudopodial nets and in debris piles. We found that Mastogloia and Cocconeis were the most prominent genera in the overall diatom assemblage. The food preference of S. dominicensis included Amphora, Berkeleya rutilans,Cocconeis, Licmophora dalmatica, Mastogloia, Odontella rhombus, Plagiogramma pulchellum var. pygmaeum, and Skeletonema.

Aging is a process characterized by accumulated oxidative damage to DNA, proteins, and lipids,which leads to the gradual degeneration of cellular activity. Mitochondria play a central role in aging because they produce both cellular energy and oxidative stress. As resultof accumulated oxidative damage, mitochondria function decays, which leads to a cellular energy deficit and compromises cellular function. Iron is an essential nutrient reequired by mitodhondria to function optimally. It has been proved that iron supplementation increases the lifespan of several yeast strains, including superoxide dismutase mutants. We are interested in finding where the iron is going and what it might be doing that is beneficial to the cell. We have used Saccharomyces cerevisiae as our molecular model of aging. Our results indicate that the extra iron is being transported into the mitochoindria.

In my thesis work, I attempted to construct a plasmid that would allow stable integration of genes into the Saccharomyces cerevisiae yeast genome under the control of the repressible TetO promoter. The yeast ACO1 gene was cloned under the control of the TetO operator and the tTA transactivator. This construct was inserted into yeast cells in order to observe the effects of aconitase overexpression on aging. Unfortunately, the transformed cells appeared incapable of aconitase expression as determined by glutamic acid auxptrophy, a phenotype of aconitase mutants. We have sequenced the pIT1ACO1 plasmid and have found many abnormalities in the promoter region. If the plasmid can be made to function as intended, the resulting yeast strain can be used in the future to determine if aconitase plays an important role in cellular aging.