Model
Digital Document
Publisher
Florida Atlantic University
Description
Gonadal (GDlI), kidney (KlK) and gill (GlB) cell lines were established
from tissues of the walking catfish using an improved procedure. These
cell lines were found to be susceptible to channel catfish virus (CCV)
infection. A virus sample (HPV) propagated for 59 passages at low
dilution in KlK cells was found to be of lower virulence for young
channel catfish than two other field strains of CCV (LPV) with fewer
in vitro passages. Injection of channel catfish with HPV protected
these fish from subsequent challenge with the more virulent LPV. Challenge of walking catfish with several different CCV samples including wild strains and Clarias-propagated virus failed to produce any
mortalities.
from tissues of the walking catfish using an improved procedure. These
cell lines were found to be susceptible to channel catfish virus (CCV)
infection. A virus sample (HPV) propagated for 59 passages at low
dilution in KlK cells was found to be of lower virulence for young
channel catfish than two other field strains of CCV (LPV) with fewer
in vitro passages. Injection of channel catfish with HPV protected
these fish from subsequent challenge with the more virulent LPV. Challenge of walking catfish with several different CCV samples including wild strains and Clarias-propagated virus failed to produce any
mortalities.
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