Thompson, Mary C.

Relationships
Member of: Graduate College
Person Preferred Name
Thompson, Mary C.
Model
Digital Document
Publisher
Florida Atlantic University
Description
This study was undertaken to determine the distribution
and possible source of Staphylococcus aureus, s. epidermidis,
other Micrococcaceae , and enterococci recovered from samples
of water collected at the following locations: the Pompano
Beach sewage outfall, the Intracoastal Waterway, 5 miles due
east of the Boca Raton Inlet, over the 65 ft Boca Raton reef,
and in 5 ft of water along the Boca Raton beach. It was also
of interest to study the feasibility of employing s. aureus
as an indicator of fecal pollution in the marine environment.
Neither staphylococci nor enterococci were recovered
from 5 samples of beach sand collected from the high tide
line to the low tide line. Only low concentrations of
staphylococci were recovered from samples collected from the
Intracoastal Waterway. The staphylococci recovered from the
marine locations do not appear to be true marine bacteria.
They do not appear to be originating from bathers, beach
washings or the Intracoastal Waterway. Sampling was inadequate
to reach definite conclusions on the origin of the
Micrococcaceae recovered in the ocean. However, these data
indicate the staphylococci originate from the Pompano Beach
sewage outfall. Staphylococcus aureus appears to be more resistant to
the conditions of the marine environment than the other
Micrococcaceae, enterococci or other sewage bacteria
recovered on Mannitol Salt Agar and m-Enterococcus Agar.
The mean per cent recovery of S. aureus increases with increased distances downstream from the outfall to the 65 ft
reef and sampling site 5 miles offshore. ~· aureus appears
to be a good indicator of fecal pollution in the marine
environment.
Survival studies of washed cells of a coagulase
positive Staphylococcus aureus run in autoclaved sea water
and autoclaved Intracoastal water gave mean decimal reduction
times of 16.6 hr and 11.4 hr, respectively.
Staphylococci were not recovered from Bryothamnion
triguetrum,Caulerpa sertularioides f. brevipes, Dasycladus
vermicularis or Padina sanctae-crucis. Ten Micrococcaceae
recovered from the ocean were not inhibited by extracts
prepared from these algae. Intact segments of these algae
possessed antibacterial activity against most, if not all
of these Micrococcaceae.