Model
Digital Document
Publisher
Florida Atlantic University
Description
The goal of this experiment is to perfect the method of trimethylsilyl derivatization of fecal steroids Coprostan-3-one and Coprostanol using pyridine & N,O-bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) containing 1% of Trimethylchlorosilane (TMCS). The extraction method for sediments involves Soxhlet extraction followed by saponification. The extraction of water samples will be done by XAD-2 resin or SPE with C18 cartridges and extracted with MeCl2. The primary instrument of this experiment was a Gas Chromatography- Mass spectroscopy (GC-MS). In addition, a Perkin-Elmer Autosystem GC coupled to a Perkin-Elmer TurboMass Gold mass spectrometer was used, with Helium as the carrier gas. Lastly, this experiment required a computer with Turbomass software version 5.4.2. to analyze samples after derivatization. The results of the GC-MS analysis showed that the methods of derivatization were correct.
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