Mariculture

Shrimp mariculture expansion in developing countries has been criticized for its ecological destruction and the resulting social conflicts. In Ecuador, shrimp pond development particularly affects rural, coastal communities. This study addresses the environmental and social effects of shrimp farming in two Ecuadorian villages, Muisne and Bunche. Interview and survey responses indicate that locals have an overall negative view of the industry. In some instances, such as increased class marginalization through reduced access to subsistence collection areas, shrimp farming directly results in conflict. However, the data also demonstrates that ponds are only one of various stressors on mangrove ecosystems. Furthermore, mariculture provides benefits to the community, including increasing employment opportunities, the local economy, and shrimp availability. In spite of these benefits, local negative perception suggests modifications to existing regulations and educational programs are necessary to minimize impacts and help the community understand the multiple factors affecting their ecosystem and livelihoods.

The commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity of natural and captive populations. While an assessment of multiple paternity is the ultimate goal of this study, for my thesis, I have completed preliminary work which includes perfecting methods of tissue collection, DNA extraction, and DNA amplification with six non-labeled polymorphic microsatellite molecular markers, using cultured Strombus gigas animals. In addition, I collected tissue and extracted DNA from three wild S. gigas adult females and their egg masses from Pelican Shoal in the Florida Keys.