Model
Digital Document
Publisher
Florida Atlantic University
Description
The current study uses a diabetic rat model to measure the effects of pulsatile
versus continuous insulin delivery on IRS-2 in the liver. The purpose is to determine if
pulsatile, compared with continuous, insulin delivery leads to reduced insulin resistance
in diabetic rats. Insulin signaling in the liver is mediated primarily through IRS-2 and
tissue responsiveness to insulin may be detected by monitoring the lRS-2 signaling
pathway. Western blots were performed to measure IRS-2 protein levels for each
delivery condition and treatment day. Results revealed that overall, the pulsatile insulin
delivery method showed a significant increase in IRS-2 levels over the continuous insulin
delivery method by treatment day 5. These findings imply that the pulsatile delivery
method, over a period of time, triggers more insulin receptor action. Conversely, the
results of the continuous delivery system show a decrease in IRS-2 levels as the number
of doses of insulin increased.
versus continuous insulin delivery on IRS-2 in the liver. The purpose is to determine if
pulsatile, compared with continuous, insulin delivery leads to reduced insulin resistance
in diabetic rats. Insulin signaling in the liver is mediated primarily through IRS-2 and
tissue responsiveness to insulin may be detected by monitoring the lRS-2 signaling
pathway. Western blots were performed to measure IRS-2 protein levels for each
delivery condition and treatment day. Results revealed that overall, the pulsatile insulin
delivery method showed a significant increase in IRS-2 levels over the continuous insulin
delivery method by treatment day 5. These findings imply that the pulsatile delivery
method, over a period of time, triggers more insulin receptor action. Conversely, the
results of the continuous delivery system show a decrease in IRS-2 levels as the number
of doses of insulin increased.
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